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Microbiology Lab Manual, 2/E
Karen Messley, Rock Valley College
Stephen Norrell

ISBN-10: 0130100293
ISBN-13:  9780130100290

Publisher:  Benjamin Cummings
Copyright:  2003
Format:  Paper; 288 pp
Published:  01/21/2003
Status: Instock


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Description

For freshman/sophomore courses in General Introductory Microbiology, and Microbiology for Allied Health Students.

This laboratory manual serves as a general introduction to the microbiology laboratory, including basic procedures and equipment. Content covers not only basic lab instructions, but background material that explains the tests, the basis of results and their applications, providing a "procedural" aspect in addition to laboratory guidance. Questions are provided with each exercise to reinforce and challenge students' understanding of basic concepts and to require students to analyze or apply the material under discussion.


Features

  • NEW - Reorganized multi-part procedures—Complex exercises have been separated into individual activities.

    • Provides students with an easier organization and procedure to follow in the lab.

  • Self-contained exercises—Include explanations of the salient points being demonstrated or tested.

    • Provides students with exercises that can serve as stand-alone labs, and a manual that will supplement and compliment any standard text.

  • Consistent sequence to each exercise—i.e., Overview; Background Information; Lab Objectives; Materials Needed; Lab Procedures; and Lab Report Form.

    • Prepares students to complete the lab with minimal additional instruction, and makes it easy for them to refer back to procedural content when studying for lab or lecture exams.

  • Flexible application of exercises—Makes them adaptable for use over various laboratory time periods.

    • Allows instructors extensive flexibility in scheduling without having to modify instructions. Enables students to adjust to any prescribed modifications with minimal confusion.

  • Logical progression to complexity of exercises—While the instructions get less comprehensive.

    • Sets the expectation that students will become more competent with each exercise, and able to problem-solve some of the needed procedures for themselves.

  • Critical thinking exercises in laboratory reports—Includes questions based on the experimental procedures and principles demonstrated in each exercise.

    • Requires students to tabulate data acquired in the exercises and critically apply information from the procedures and results to other situations.


New To This Edition

  • Reorganized multi-part procedures—Complex exercises have been separated into individual activities.

    • Provides students with an easier organization and procedure to follow in the lab.


Table of Contents

I. MICROSCOPIC TECHNIQUES.

 1. Introduction to Microscopy.

 2. Preparation of Smears and Simple Stains.

 3. Wet Mounts and Hanging Drops.

 4. The Negative Stain.

 5. Differential Staining Procedures: Gram Stain, Acid-Fast Stain.

 6. Additional Differential Staining Procedures: Metachromatic-Granules, Capsule, Endospore and Flagella Stains.

II. MICROBIAL DIVERSITY.

 7. Ubiquity of Microorganisms.

 8. Survey of Microorganisms.

III. MICROBIAL CULTIVATION TECHNIQUES.

 9. Isolation Techniques: Streak Plate.

10. Isolation Techniques: Pour Plates.

11. Oxygen Requirements of Microorganisms.

12. Physical Growth Requirements.

13. Selective and Differential Media.

14. Physiological Characteristics of Bacteria: Carbohydrate Metabolism.

15. Physiologic Characteristics of Bacteria: Reactions of Nitrogen Metabolism.

16. Physiologic Characteristics of Bacteria: Miscellaneous Reactions.

17. Multiple Test Media.

IV. IDENTIFICATION TECHNIQUES.

18. Development and Use of Diagnostic Keys.

19. Use of Diagnostic Keys: Bacterial Unknowns.

20. Rapid Diagnostic Tests: Profile Analysis.

V. MICROBIAL GROWTH.

21. Microbial Population Counts: Microscopic Methods.

22. Microbial Population Counts: Viable Cell Counts.

VI. MICROBIAL CONTROL.

23. Physical Control Methods.

24. UV Light as an Antimicrobial Agent.

25. Assay of Antimicrobial Agents: Disc-Diffusion Methods.

26. Assay of Antimicrobial Agents: Use-Dilution Methods.

27. Antibiotic Sensitivity Testing: The Kirby-Bauer Procedure.

28. Detection of Mutant Strains of Bacteria.

VII. CLINICAL MICROBIOLOGY.

29. Epidemiology.

30. Serological Reactions.

31. The Collection and Transport of Clinical Specimen.

32. Throat Cultures.

33. Urinary Tract Cultures.

34. Gastrointestinal Tract Cultures.

35. Lactobacillus Activity.

VIII. VIROLOGY.

36. Viral Population Counts: Plaque Counting and Plaque Morphology.

IX. APPLIED MICROBIOLOGY.

37. Food Microbiology.

38. Water Analysis: Standard Methods.

39. Water Analysis: Membrane Filter Techniques.

Appendix A: Writing a Lab Report.

Appendix B: Cultures Used.

Appendix C: Media, Stains, Reagents, and Supplies.



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This laboratory manual recognizes that microbiology students need to establish a firm foundation in underlying principles as well as participate in realistic and practical experiments. Each laboratory exercise report form includes questions relating to the laboratory exercise that require relating background material to the test results, interpretation of the test results, and critical thinking/application questions that require the student to draw upon their own experience.

Topics Include:
  • Preparation of Smears and Simple Stains
  • Ubiquity of Microorganisms
  • Isolation Techniques
  • Detection of Mutant Strains of Bacteria
  • Epidemiology
  • Water Analysis

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For the Biology & Microbiology Discipline

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©2004  |  Benjamin Cummings  |  Paper; 80 pp  |  Instock
ISBN-10: 0131136747  |  ISBN-13: 9780131136748
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